The PrimeScript RT Reagent Kit (Perfect Real Time) includes PrimeScript reverse transcriptase for strong strand-displacement activity and efficient synthesis of cDNA for real-time PCR. PrimeScript RTase is a modified, recombinant MMLV (Moloney murine leukemia virus) reverse transcriptase and is verified to be RNase H-minus.
PrimeScript RTase is also robust, versatile, and can be used for reverse transcription of virtually any RNA template, including templates that have a high degree of secondary structure or are GC rich. Because of the excellent extension capability of PrimeScript, cDNA synthesis reactions can be performed at 42°C, minimizing the risk of RNA degradation that often occurs at higher temperatures.buffer
The PrimeScript RT Reagent Kit (Perfect Real Time) includes PrimeScript reverse transcriptase for strong strand-displacement activity and efficient synthesis of cDNA for real-time PCR. PrimeScript RTase is a modified, recombinant MMLV (Moloney murine leukemia virus) reverse transcriptase and is verified to be RNase H-minus.
PrimeScript RTase is also robust, versatile, and can be used for reverse transcription of virtually any RNA template, including templates that have a high degree of secondary structure or are GC rich. Because of the excellent extension capability of PrimeScript, cDNA synthesis reactions can be performed at 42°C, minimizing the risk of RNA degradation that often occurs at higher temperatures.buffer